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sfk py527 cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sfk py527 cell signaling technology
    Antibodies and Dilutions Used for IHC and Western Blot Analysis
    Sfk Py527 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sfk+py527+cell+signaling+technology/Phospho-Src+(Tyr527)+Antibody/pmc06983918-171-101-102
    Average 96 stars, based on 370 article reviews
    sfk py527 cell signaling technology - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers"

    Article Title: Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2019.10.017

    Antibodies and Dilutions Used for IHC and Western Blot Analysis
    Figure Legend Snippet: Antibodies and Dilutions Used for IHC and Western Blot Analysis

    Techniques Used: Western Blot

    Related Articles

    Western Blot:

    Article Title: Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers
    Article Snippet: Reagents and Antibodies The following primary antibodies were used: SFK_pY416 number 2101, SFK_pY527 number 2105, Src number 2108, Lyn number 2732, Lck number 2752, CDCP1 pY734 number 9050, CDCP1 pY743 number 14965, CDCP1 number 13794, CDCP1 number 4115, PKCδ pY311 number 2055, PKCδ number 2058, AMP-activated protein kinase alpha (AMPKα) pT172 number 2535, AMPKα number 2532, N-cadherin number 13116, Slug number 9585, PD1 number 86163, and PD-L1 number 13684 (Cell Signaling Technology, Danvers, MA); PKCδ pY311 number ab76181 and FOXA1 number ab173287 (Abcam, Cambridge, MA); vimentin number MA511883 and cytokeratin 5/6 (CK5/6) number 180267 (Thermo Fisher Scientific, Waltham, MA); β-actin number A5441 (Millipore Sigma, St. Louis, MO); vimentin number M0725 (Agilent, Santa Clara, CA); Sry-box 10 (Sox10) number BSB2583 (Bio SB, Santa Barbara, CA); CD3 number NCL-L-CD3-565 (Leica Biosystems, Wetzlar, Germany); and CTLA4 number sc-376016 (Santa Cruz Biotechnology, Dallas, TX). .. Antibody dilutions for each method are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Antibody information, manufacturer/catalog no. Western blot dilution IHC dilution Antigen retrieval ∗ FOXA1 Abcam/ab173287 NA 1:4000 1 Vimentin Agilent/M0725 NA 1:2000 2 Sox10 Bio SB/BSB2583 NA 1:100 1 CK5/6 Thermo Fisher Scientific/180267 NA 1:50 2 CDCP1 Cell Signaling Technology/4115 1:1000 1:100 1 CDCP1_pY743 Cell Signaling Technology/14965 1:1000 1:800 1 CDCP1_pY734 Cell Signaling Technology/9050 1:1000 NA NA Src Cell Signaling Technology/2108 1:1000 NA NA Lck Cell Signaling Technology/2752 1:1000 NA NA Lyn Cell Signaling Technology/2732 1:1000 NA NA SFK_pY416 Cell Signaling Technology/2101 1:1000 1:200 1 SFK_pY527 Cell Signaling Technology/2105 1:1000 NA NA PKCδ Cell Signaling Technology/2058 1:1000 NA NA PKCδ_pY311 Abcam/ab76181 1:2500 1:3000 1 N-cadherin Cell Signaling Technology/13116 NA 1:200 1 Slug Cell Signaling Technology/9585 NA 1:100 1 AMPKα Cell Signaling Technology/2532 1:1000 NA NA AMPKα_pT172 Cell Signaling Technology/2535 1:1000 NA NA β-Actin Leica Biosystems/A5441 1:5000 NA NA CD3 Novocastra/NCL-L-CD3-565 NA 1:50 2 PD1 Cell Signaling Technology/86163 NA 1:200 2 PD-L1 Cell Signaling Technology/13684 NA 1:400 2 CTLA4 Santa Cruz Biotechnology/sc-376016 NA 1:400 2 Open in a separate window AMPK, AMP-activated protein kinase; CDCP, CUB-domain containing protein; CK5/6, cytokeratin 5/6; CTLA4, cytotoxic T-lymphocyte protein 4; FOXA1, forkhead box protein A1; IHC, immunohistochemistry; Lck, tyrosine-protein kinase Lck; Lyn, tyrosine-protein kinase Lyn; NA, not applicable; PD1, programmed cell death protein 1; PD-L1, programmed cell death 1 ligand 1; PKC, protein kinase C; Sox10, Sry-box transcription factor 10. .. ∗ Antigen retrieval 1: heat 20 minutes off-line in Leica ER2 buffer in steamer; repeat online with Leica ER2 buffer for 20 minutes.

    Immunohistochemistry:

    Article Title: Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers
    Article Snippet: Reagents and Antibodies The following primary antibodies were used: SFK_pY416 number 2101, SFK_pY527 number 2105, Src number 2108, Lyn number 2732, Lck number 2752, CDCP1 pY734 number 9050, CDCP1 pY743 number 14965, CDCP1 number 13794, CDCP1 number 4115, PKCδ pY311 number 2055, PKCδ number 2058, AMP-activated protein kinase alpha (AMPKα) pT172 number 2535, AMPKα number 2532, N-cadherin number 13116, Slug number 9585, PD1 number 86163, and PD-L1 number 13684 (Cell Signaling Technology, Danvers, MA); PKCδ pY311 number ab76181 and FOXA1 number ab173287 (Abcam, Cambridge, MA); vimentin number MA511883 and cytokeratin 5/6 (CK5/6) number 180267 (Thermo Fisher Scientific, Waltham, MA); β-actin number A5441 (Millipore Sigma, St. Louis, MO); vimentin number M0725 (Agilent, Santa Clara, CA); Sry-box 10 (Sox10) number BSB2583 (Bio SB, Santa Barbara, CA); CD3 number NCL-L-CD3-565 (Leica Biosystems, Wetzlar, Germany); and CTLA4 number sc-376016 (Santa Cruz Biotechnology, Dallas, TX). .. Antibody dilutions for each method are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Antibody information, manufacturer/catalog no. Western blot dilution IHC dilution Antigen retrieval ∗ FOXA1 Abcam/ab173287 NA 1:4000 1 Vimentin Agilent/M0725 NA 1:2000 2 Sox10 Bio SB/BSB2583 NA 1:100 1 CK5/6 Thermo Fisher Scientific/180267 NA 1:50 2 CDCP1 Cell Signaling Technology/4115 1:1000 1:100 1 CDCP1_pY743 Cell Signaling Technology/14965 1:1000 1:800 1 CDCP1_pY734 Cell Signaling Technology/9050 1:1000 NA NA Src Cell Signaling Technology/2108 1:1000 NA NA Lck Cell Signaling Technology/2752 1:1000 NA NA Lyn Cell Signaling Technology/2732 1:1000 NA NA SFK_pY416 Cell Signaling Technology/2101 1:1000 1:200 1 SFK_pY527 Cell Signaling Technology/2105 1:1000 NA NA PKCδ Cell Signaling Technology/2058 1:1000 NA NA PKCδ_pY311 Abcam/ab76181 1:2500 1:3000 1 N-cadherin Cell Signaling Technology/13116 NA 1:200 1 Slug Cell Signaling Technology/9585 NA 1:100 1 AMPKα Cell Signaling Technology/2532 1:1000 NA NA AMPKα_pT172 Cell Signaling Technology/2535 1:1000 NA NA β-Actin Leica Biosystems/A5441 1:5000 NA NA CD3 Novocastra/NCL-L-CD3-565 NA 1:50 2 PD1 Cell Signaling Technology/86163 NA 1:200 2 PD-L1 Cell Signaling Technology/13684 NA 1:400 2 CTLA4 Santa Cruz Biotechnology/sc-376016 NA 1:400 2 Open in a separate window AMPK, AMP-activated protein kinase; CDCP, CUB-domain containing protein; CK5/6, cytokeratin 5/6; CTLA4, cytotoxic T-lymphocyte protein 4; FOXA1, forkhead box protein A1; IHC, immunohistochemistry; Lck, tyrosine-protein kinase Lck; Lyn, tyrosine-protein kinase Lyn; NA, not applicable; PD1, programmed cell death protein 1; PD-L1, programmed cell death 1 ligand 1; PKC, protein kinase C; Sox10, Sry-box transcription factor 10. .. ∗ Antigen retrieval 1: heat 20 minutes off-line in Leica ER2 buffer in steamer; repeat online with Leica ER2 buffer for 20 minutes.



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    sfk py527 cell signaling technology  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc sfk py527 cell signaling technology
    Antibodies and Dilutions Used for IHC and Western Blot Analysis
    Sfk Py527 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sfk+py527+cell+signaling+technology/Phospho-Src+(Tyr527)+Antibody/pmc06983918-171-101-102
    Average 96 stars, based on 1 article reviews
    sfk py527 cell signaling technology - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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    Antibodies and Dilutions Used for IHC and Western Blot Analysis

    Journal: The American Journal of Pathology

    Article Title: Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers

    doi: 10.1016/j.ajpath.2019.10.017

    Figure Lengend Snippet: Antibodies and Dilutions Used for IHC and Western Blot Analysis

    Article Snippet: Antibody dilutions for each method are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Antibody information, manufacturer/catalog no. Western blot dilution IHC dilution Antigen retrieval ∗ FOXA1 Abcam/ab173287 NA 1:4000 1 Vimentin Agilent/M0725 NA 1:2000 2 Sox10 Bio SB/BSB2583 NA 1:100 1 CK5/6 Thermo Fisher Scientific/180267 NA 1:50 2 CDCP1 Cell Signaling Technology/4115 1:1000 1:100 1 CDCP1_pY743 Cell Signaling Technology/14965 1:1000 1:800 1 CDCP1_pY734 Cell Signaling Technology/9050 1:1000 NA NA Src Cell Signaling Technology/2108 1:1000 NA NA Lck Cell Signaling Technology/2752 1:1000 NA NA Lyn Cell Signaling Technology/2732 1:1000 NA NA SFK_pY416 Cell Signaling Technology/2101 1:1000 1:200 1 SFK_pY527 Cell Signaling Technology/2105 1:1000 NA NA PKCδ Cell Signaling Technology/2058 1:1000 NA NA PKCδ_pY311 Abcam/ab76181 1:2500 1:3000 1 N-cadherin Cell Signaling Technology/13116 NA 1:200 1 Slug Cell Signaling Technology/9585 NA 1:100 1 AMPKα Cell Signaling Technology/2532 1:1000 NA NA AMPKα_pT172 Cell Signaling Technology/2535 1:1000 NA NA β-Actin Leica Biosystems/A5441 1:5000 NA NA CD3 Novocastra/NCL-L-CD3-565 NA 1:50 2 PD1 Cell Signaling Technology/86163 NA 1:200 2 PD-L1 Cell Signaling Technology/13684 NA 1:400 2 CTLA4 Santa Cruz Biotechnology/sc-376016 NA 1:400 2 Open in a separate window AMPK, AMP-activated protein kinase; CDCP, CUB-domain containing protein; CK5/6, cytokeratin 5/6; CTLA4, cytotoxic T-lymphocyte protein 4; FOXA1, forkhead box protein A1; IHC, immunohistochemistry; Lck, tyrosine-protein kinase Lck; Lyn, tyrosine-protein kinase Lyn; NA, not applicable; PD1, programmed cell death protein 1; PD-L1, programmed cell death 1 ligand 1; PKC, protein kinase C; Sox10, Sry-box transcription factor 10.

    Techniques: Western Blot